lifesciences
Innopsys

Research

Innopsys products are perfectly suited for all applications on the Omics approaches based on microarrays; either glycomics, proteomics or genomics.

genomique

Microarrays have revolutionized functional genomics fields. Using DNA or RNA arrays it is now possible to define genomic signatures face to different stimuli such as drug treatment or disease. Gene expression microarrays and miRNA arrays are used to define changes in the expression of thousands of genes at once, while Chip-on-Chip microarrays are useful to detect pairing sites for transcription factors along the genome, such is the case of human promoter microarrays.

InnoScan scanner models are ideal tools for all these research fields, for low and medium density arrays, such as BAC or oligo microarrays, the InnoScan 710 and the InnoScan 300 scanners are suitable for two or single color microarrays, respectively. While for HD microarrays such as 8x60K or 4x180K array formats, the InnoScan 910 scanner offer users the highest resolution on the market (1µm/pixel) combining speed and precision for a reliable result.

glycomics

Glycans are complex forms of carbohydrates. In the same way as proteins, glycans are considered as final effectors in the cellular response to stimuli. Moreover, glycans play an essential role in cell-cell communication and in cell recognition through the immunological system. Glycomics is a new field which aims to define the structure and function of glycans and carbohydrates in living organisms.

Glycan arrays such as lectin arrays are useful tools for defining either protein modification by sugars, or the interaction between glycans with proteins, phospholipids, etc. FITC-labelled glycans are often used as detection probes. The InnoScan 1100 AL is a good option for detecting FITC and other dyes on the blue channel, together with classical green and red channels.

proteomique

Proteins are considered as the effectors on cell response to internal or external stimuli such as disease or drug treatment. Proteomics aims at defining those protein signatures involved in cell adaptation or reaction to a specific event. Antibody microarrays are useful tools for proteome studies since they can define either the protein expression or the protein activity by defining their activation status (glycosylation, phosphorylation). The InnoScan 710 and the InnoScan 300 are ideal tools to scan two or one color protein arrays, respectively.

Reverse Phase Protein arrays (RPPA) are excellent tools to validate protein biomarker expression on large scale cohorts. In this technology hundreds of samples are immobilized in specific substrates such as nitrocellulose slides, then protein of interest are detected by validated antibodies coupled to fluorescence. The InnoScan 710-IR scanner has been specifically conceived to scan nitrocellulose-based RPPA assays, its infrared detection avoids nitrocellulose auto-fluorescence giving increased sensitivity and dynamic range.

High density peptide arrays are nowadays important tools in proteomics to characterize antibody recognition sites (epitope mapping) either in normal conditions or during infection. Next generation peptide arrays are HD arrays containing thousands of probes sizing less than 13µm. For these arrays the InnoScan 910 and the InnoScan 1100 AL scanner models have the resolution and dynamic range to warranty robustness and reliability on scanning HD and ultra-HD peptide arrays, respectively.

cell and tissue imaging application 600x200

Cell evolution and response to environmental stimuli depends on the interaction of several proteins at once. To deal with such complexity, multiplexed approaches are indispensable. Cell microarrays (CMA) and tissue microarrays (TMA) allow to perform multiplexed assays for protein expression and biomarker localization on cells and tissue samples, respectively. In addition to multiplexing, quantitative high throughput fluorescence imaging has been shown to generate more data in less time.

The InnoScan 1100 AL is the perfect tool for imaging CMAs and TMAs as it allows users to easily acquire high-quality whole slide images. With its high resolution (0.5µm/pixel), it allows users to reach a sub-cellular level of analysis.

Equipped with three independent photomultipliers for signal detection, the InnoScan 1100 AL allows highly sensitive detection of three biomarkers simultaneously. Its high sensitivity allows for a quantitative analysis of biomarker expression in tissue and cell samples.

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