lifesciences
Innopsys

Infrared detection decreases nitrocellulose autofluorescence and improves RPPA assays signal-to-noise ratio over visible wavelength detection

Reverse Phase Protein Arrays (RPPAs) are a specific type of protein arrays consisting of printed cell lysates useful for cell signaling and biomarker analysis. RPPAs are mainly built on nitrocellulose film slides as they have high binding capacity and viability for native proteins. Until now, RPPA assays with fluorescence detection were limited in sensitivity by high intrinsic background fluorescence normally produced by the nitrocellulose, however, it is now possible to increase the Signal to Noise Ratio using Near Infrared (NIR) detection with the InnoScan 710-IR scanner. Results show an increase up to 3 fold in signal to noise ratio when compared with traditional fluorescence scanning, mainly due to lower intrinsic background fluorescence in the NIR.

 

Marie-Laure Schneider1, Adriana Marquez-Lagraulet2, Richard Pasquesi3, Michael Shultz4

  • 1,3 Innopsys Inc. Chicago, IL
  • 2 Innopsys, Parc Activestre 31270 Carbonne, France
  • 4 Grace Bio-Labs Inc, Bend, OR

 

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