lifesciences
Innopsys

Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes

Abstract

With the increasing need for understanding antibody specificity in antibody and vaccine research, pepscan assays provide a rapid method for mapping and profiling antibody responses to continuous epitopes. We have developed a relatively low-cost method to generate peptide microarray slides for studying antibody binding. Using a setup of an IntavisAG MultiPep RS peptide synthesizer, a Digilab MicroGrid II 600 microarray printer robot, and an InnoScan 1100 AL scanner, the method allows the interrogation of up to 1536 overlapping, alanine-scanning, and mutant peptides derived from the target antigens. Each peptide is tagged with a polyethylene glycol aminooxy terminus to improve peptide solubility, orientation, and conjugation efficiency to the slide surface.

Author

McBride R1, Head SR1, Ordoukhanian P2, Law M1

  • 1. DNA Array Core, Department of Immunology and Microbial Science, The Scripps Research Institute
  • 2. Protein and Nucleic Acid CoreFacility, Department ofImmunology and Microbial Science, The Scripps Research Institute

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