lifesciences
Innopsys

O-156 Snp array -based combination of copy number and genotype analyses to determine chromosomal imbalances in human blastomeres

Hum. Reprod. (2010) 25 (suppl 1): ii61-ii63. This article appears in: Abstracts of the 26th Annual Meeting of the European Society of Human Reproduction and Embryology Rome, Italy, 27-30 June 2010 doi: 10.1093/humrep/de.25.s1.42  


SESSION  42: Preimplantation Genetic Diagnosis
O-156 Snp array -based combination of copy number and genotype analyses to determine chromosomal imbalances in human blastomeres

C.M.J. Uum van1, S.J.C. Stevens1, J.C.F.M. Dreesen1,2, M. Drusedau2, H.J.M. Smeets1,2, H.T.M. Hollander-Crombach1, J.P.M. Geraedts1,2, J.J.M. Engelen1,2 and E. Coonen1,3
Author Affiliations
1.Academic Hospital Maastricht, Clinical Genetics, Maastricht, The Netherlands 
2.Academic Hospital Maastricht, Research Institute GROW, Maastricht, The Netherlands 
3.Academic Hospital Maastricht, Obstetrics & Gynaecology IVF laboratory, Maastricht, The Netherlands   

Abstract

Introduction:
Whole genome amplification (WGA) and subsequent DNA analysis on high resolution array platforms are promising tools in the development of universal, off-the-shelf PGD protocols that may replace FISH and multiplex PCR methods. SNP arrays have the advantage over oligo-array CGH that loci can be interpreted for both chromosomal copy number and genotypic data. We developed a WGA-SNP array method for the detection of chromosomal imbalances in single blastomeres by SNP copy number. In addition, we tested feasibility to confirm these copy number results by SNP genotype data.

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