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Anti-heat shock protein autoantibody profiling in breast cancer using customized protein microarray

Abstract

Heat shock proteins (HSPs) are over-expressed in a wide range of human cancers. It results in the stimulation of the immune system and consequently in elevated concentration of anti-HSP autoantibodies. Elevated anti-HSP autoantibodies were found in breast cancer patients, and they are associated with tumor metastasis. Therefore, screening these autoantibodies could be of diagnostic and prognostic values. Protein microarrays have already demonstrated their great potential as a diagnostic tool. However, protein diversity requires optimization of the microarray fabrication to achieve high sensitivity and specificity. In this study, seven HSPs were immobilized on six different surface chemistries. After evaluation and optimization with purified antibodies of the six surface chemistries, two surfaces were selected to detect anti-HSP autoantibodies in breast cancer sera. Multiplex detection of anti-HSP autoantibodies allowed discrimination of breast cancer patients (50) from healthy controls (26) with a sensitivity of 86 % and a specificity of 100 %.

Authors:

Liu Shi1, Thomas Gehin1, Yann Chevolot1, Eliane Souteyrand1, Alain Mangé2, Jérôme Solassol2, Emmanuelle Laurenceau1

  • 1. INSTITUT DES Nanotechnologies de Lyion INL-UMR5270, CNRS, Ecole Centrale de Lyon, Université de Lyon, 69134 Ecully, France
  • 2. Institut de Recherche en Cancérologie de Montpellier INSERM U1194, Campus Val d’Aurelle, 34298 Montpellier, France

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