lifesciences
Innopsys

iSpOD: probe design for functional DNA microarrays

Abstract:

MOTIVATION:

The use of DNA microarrays allows the monitoring of the extreme microbial diversity encountered in complex samples like environmental ones as well as that of their functional capacities. However, no probe design software currently available is adapted to easily design efficient and explorative probes for functional gene arrays.

RESULTS:

We present a new efficient functional microarray probe design algorithm called HiSpOD (High Specific Oligo Design). This uses individual nucleic sequences or consensus sequences produced by multiple alignments to design highly specific probes. Indeed, to bypass crucial problem of cross-hybridizations, probe specificity is assessed by similarity search against a large formatted database dedicated to microbial communities containing about 10 million coding sequences (CDS). For experimental validation, a microarray targeting genes encoding enzymes involved in chlorinated solvent biodegradation was built. The results obtained from a contaminated environmental sample proved the specificity and the sensitivity of probes designed with the HiSpOD program

Credits:

Eric Dugat-Bony1,2, Mohieddine Missaoui3,4, Eric Peyretaillade2,5, Corine Biderre-Petit1,2, Ourdia Bouzid1,2, Christophe Gouinaud3,4, David Hill3,4, Pierre Peyret2,5

  • 1. Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes : Génome et Environnement, BP 10448, F63000, Clermont-Ferrand
  • 2. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand
  • 3. Clermont Université, Université Blaise Pascal, LIMOS, BP 1044, F-63000 Clermont-Ferrand
  • 4. UMR CNRS 6158, LIMOS, F-63173 Aubière
  • 5. Clermont Université, Université d’Auvergne, Laboratoire Microorganismes : Génome et Environnement, BP 10448, F63000, Clermont-France,

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