lifesciences
Innopsys

NIR RPPA detection together with dynamic range extension (XDR) function in the InnoScan 710-IR scanner makes RPPA detection cheaper by 90%

Reverse Phase Protein Arrays (RPPA) also named cell lysate arrays have been shown to be useful tools for biomarker evaluation in large patient cohorts. Using validated antibodies, biomarkers of cell faith can be followed in a single experiment. One of the challenges of this technology is the dynamic range, since biomarker expression levels can vary from fento-molar (fM) to micro-molar (µM) concentration in the same cohort. Several technologies have been developed to follow the expression of biomarkers. Fluorescent detection has been demonstrated to be more sensitive than colorimetric and chemiluminiscence detection. However, all these technologies have to be adapted to overcome the challenge of a limited dynamic range. One solution is to perform serial dilutions of samples. However, this increases RPPA complexity and makes data interpretation quite tedious. Moreover, serial dilutions increase RPPA costs since several slides per experiment are needed. Here we demonstrate how by using the dynamic range extension (XDR) function in the InnoScan 710-IR scanner, RPPA costs are dramatically diminished when switching from colorimetric to fluorescence detection.

 

Adriana Lagraulet1, Richard Pasquesi2

  • 1 Innopsys, Parc Activestre, Carbonne – France
  • 2 Innopsys Inc., Chicago IL www.innopsys.com

 

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