Effect of pattern size and pitch for the immobilization of cancer cells on biochemically-patterned glass slides fabricated with the InnoStamp40


Mastering the adhesion landscape of living cells on a surface is emerging as a new tool for investigating a lot of fundamental mechanisms of cell biology such as shape control, differentiation, division, polarity or motility[1-12]. The common point to all these studies is the production of micro-patterns of various shapes and dimensions along which adherent cells are immobilized in a deterministic way. Cell adhesion controlling through micro-patterning allows biologists to follow up cell response to different adhesion stimuli. Because automated patterning technologies allow reproducing identical precise patterns along well-arranged periodical arrays, these experimental observations can be done systematically over large population of cells thus reaching a high level of representativeness.

The micro-contact printing of proteins of the Extra Cellular Matrix (ECM) on conventional glass slides or coverslips turned out to be an efficient method for fabricating these micro-patterned cell culture substrates. However, manual processing fails in generating reproducible patterns over large surfaces, limiting the real potential of this new approach. In order to bring micro-patterning to a level where it could become a standard tool in cell biology, we propose to automatize the printing process of ECM proteins on cell culture substrates. A fine control of the stamping parameters is a sine qua none condition to print in one step reproducible patterns of different shape, size and pitch.

In this note, we present a dedicated study in which different pattern sizes and gaps for the deterministic immobilization of cancer cells were screened.


Jean-christophe CAU1


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