lifesciences
Innopsys

Reproducibility of automated protein microcontact printing with Innostamp40

Abstract

Microcontact printing (μCP) is a soft lithography technique for the creation of micrometric and nanometric molecular patterns. When used for the production of living cell arrays, these patterns are composed of extracellular matrix (ECM) proteins and can be printed on glass slides, Petri dish, PDMS layers, hydrogels or other kind of materials and formats. The aim is to control the adhesive pattern of the cells to investigate key cellular mechanisms such as differentiation, migration, adhesion, polarity or mechanosensing. This technique is applicable to various types of living cells: stem cells, cancer cells, neural cells, immune cells, epithelial cells [1-8]; and allows biologists to create precise conditions of adhesion over a large population of cells under investigation.

Credit

Lucas Malpartida1, Emmanuelle Trevisiol1, Christophe Vieu1, Jean-Christophe Cau2

  1. Biosoft, LAAS-CNRS, 7 avenue du colonel Roche, Toulouse, France
  2. Innopsys, Parc activestre, Carbonne – France – contact@innopsys.fr

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