lifesciences
Innopsys

Chicken GRIFIN: A homodimeric member of the galectin network with canonical properties and a unique expression profile

Occurrence of the adhesion/growth-regulatory galectins as family sets the challenge to achieve a complete network analysis. Along this route taken for a well-suited model organism (chicken), we fill the remaining gap to characterize its seventh member known from rat as galectin-related inter-fiber protein (GRIFIN) in the lens. Its single-copy gene is common to vertebrates, with one or more deviations from the so-called signature sequence for ligand (lactose) contact. The chicken protein is a homodimeric agglutinin with capacity to bind β-galactosides, especially the histo-blood group B tetrasaccharide, shown by solid-phase/cell assays and a glycan microarray. Mass spectrometric identification of two lactose-binding peptides after tryptic on-bead fragmentation suggests an interaction at the canonical region despite a sequence change from Arg to Val at the site, which impairs reactivity of human galectin-1. RT-PCR and Western blot analyses of specimen from adult chicken organs reveal restriction of expression to the lens, here immunohistochemically throughout its main body. This report sets the stage for detailed structure-activity studies to define factors relevant for affinity beyond the signature sequence and to perform the first complete network analysis of the galectin family in developing and adult organs of a vertebrate.

Credits:

Gabriel Garcia Caballeroa, Hebert Kaltnera, Malwina Michalakb, Nadezhda Shilovac, Michelle Ygresd, Sabine Andréa, Anna-Kristin Ludwiga, Joachim C. Manniga, Sebastian Schmidta, Martina Schnölzere, Nicolai V. Bovinc, Dietmar Reuschd, Jürgen Kopitzb, Hans-Joachim Gabiusa

  • a. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539 Munich, Germany
  • b. Department of Applied Tumor Biology, Institute of Pathology, Medical School of the Ruprecht-Karls-University, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany
  • c. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya, Moscow, Russia
  • d. Pharma Biotech Development Penzberg, Roche Diagnostics GmbH, 82377 Penzberg, Germany
  • e. Genomics and Proteomics Core Facility, German Cancer Research Center, 69120 Heidelberg, Germany

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