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miR-200c affects the mRNA expression of E-cadherin by regulating the mRNA level of TCF8 during post-natal epididymal development in juvenile rats

miR-200c affects the mRNA expression of E-cadherin by regulating the mRNA level of TCF8 during post-natal epididymal development in juvenile rats

Junfeng Wang and Kangcheng Ruan State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Abstract

The unique temporal expression pattern of miR-200c in epididymis during post-natal development in juvenile rats was revealed by our home-made miRNA microarray in this paper. It was found that miR200c expressed in the lowest level at Day 7 and then increased to the highest at Day 36 followed by a dramatic decrease. The pattern was exactly inverse to that of mRNA expression of transcription factor 8 (TCF8) revealed by quantitative real-time polymerase chain reaction (qRT-PCR), providing an extra evidence that TCF8 is one degradation target of miR-200c even in epididymis. Moreover, the qRT-PCR study on expression of E-cadherin and interleukin-2 indicated that miR-200c does exert an obvious effect on the mRNA expression of E-cadherin by directly regulating the mRNA level of TCF8, although the effect on interleukin-2 is not obvious as on E-cadherin, which implicates that interleukin-2 may be also regulated by other factors besides TCF8 in rat epididymis.

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