lifesciences
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Comparative evaluation of effectiveness of IAVchip DNA microarray in influenza A diagnosis

Abstract:

The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8-10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes.

Credits:

Sultankulova KT1, Chervyakova OV1, Kozhabergenov NS1, Shorayeva KA1, Strochkov VM1, Orynbayev MB1, Sandybayev NT1, Sansyzbay AR1, Vasin AV2

  • 1. Research Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, Kazakhstan.
  • 2. Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Prof. Popov Street 15/17, Saint Petersburg 197376, Russia

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