lifesciences
Innopsys

Protein-based nanotoxicology assessment strategy

Abstract:

The nanomaterial community calls for standardized in vitro assays to determine nanoparticle toxicity in the effort to reduce the number of in vivo validation experiments. We demonstrate that chip-based protein detection is suitable for assessing toxicity and may complement traditional assays to improve selection of primary hits for subsequent analysis. As nanodrug mimics, we analyzed the effect of transiently transfected siRNAs in MCF7 breast cancer cells and normal MCF12A breast cells, resembling a differential screen. As a measure of cytotoxicity, we determined cell viability as well as protein expression of glyceraldehyde-3-phosphate dehydrogenase, transferrin receptor, and the proliferation marker Ki67. The evaluation of cell lethality and protein expression unraveled cellular effects overseen by one method alone

Credits:

Marlene Pedersen Elnegaard1, Markus List1, Helle Christiansen2, Steffen Schmidt2, Jan Mollenhauer3, Ines Block3,4.

  • 1. Lundbeckfonden Center of Excellence in Nanomedicine NanoCAN, University of Southern Denmark, Odense, Denmark
  • 2. Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark
  • 3. Institute of Clinical Research, University of Southern Denmark, Odense, Denmark

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