lifesciences
Innopsys

Glycoprofiling as a novel tool in serological assays of systemc sclerosis: a comparative study with three bioanalytical methods

Abstract:

Systemic sclerosis (SSc) is an autoimmune disease seriously affecting patient’s quality of life. The heterogeneity of the disease also means that identification and subsequent validation of biomarkers of the disease is quite challenging. A fully validated single biomarker for diagnosis, prognosis, disease activity and assessment of response to therapy is not yet available. The main aim of this study was to apply an alternative assay protocol to the immunoassay-based analysis of this disease by employment of sialic acid recognizing lectin Sambucus nigra agglutinin (SNA) to glycoprofile serum samples. To our best knowledge this is the first study describing direct lectin-based glycoprofiling of serum SSc samples. Three different analytical methods for glycoprofiling of serum samples relying on application of lectins are compared here from a bioanalytical point of view including traditional ELISA-like lectin-based method (ELLA), novel fluorescent lectin microarrays and ultrasensitive impedimetric lectin biosensors. Results obtained by all three bioanalytical methods consistently showed differences in the level of sialic acid present on glycoproteins, when serum from healthy people was compared to the one from patients having SSc. Thus, analysis of sialic acid content in human serum could be of a diagnostic value for future detection of SSc, but further work is needed to enhance selectivity of assays for example by glycoprofiling of a fraction of human serum enriched in antibodies for individual diagnostics.

Credits:

Katrlik1, Alica Vikartovska1, Jaroslav Filip1, Peter Kasak2,3, Anita Adicsova-Eckstein4, Jaroslav Monsnacek4, Josev Lukac5, Jozef Rovensky5, Richard Imrich6, Jan Tkac1

  • 1. Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta9, Bratislava 845 38, Slovakia
  • 2. Center for Advanced Materials, Qatar University, P.O. Box 2713, Doha, Qatar
  • 3. Department for Biomaterial Research, Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 845 41, Slovakia
  • 4. Departmen of Synthesis and Characterization of Polymers, Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 845 41, Slovakia
  • 5. National Institute of Rheumatic Diseases, Nabr. I. Krasku 4, Piet’any 921 12, Slovakia
  • 6. Laboratory of Human Endocrinology, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, Bratislava 833 06, Slovakia

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