lifesciences
Innopsys

A Conserved Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation

Global inspection of plant genomes identifies genes maintained in low copies across taxa and under strong purifying selection, which are likely to have essential functions. Based on this rationale, we investigated the function of the low-duplicated CYP715 cytochrome P450 gene family that appeared early in seed plants and evolved under strong negative selection. Arabidopsis CYP715A1 showed a restricted tissue-specific expression in the tapetum of flower buds and in the anther filaments upon anthesis. cyp715a1 insertion lines showed a strong defect in petal development, and transient alteration of pollen intine deposition. Comparative expression analysis revealed the downregulated expression of genes involved in pollen development, cell wall biogenesis, hormone homeostasis, and floral sesquiterpene biosynthesis, especially TPS21 and several key genes regulating floral development such as MYB21, MYB24, and MYC2. Accordingly, floral sesquiterpene emission was suppressed in the cyp715a1 mutants. Flower hormone profiling, in addition, indicated a modification of gibberellin homeostasis and a strong disturbance of the turnover of jasmonic acid derivatives. Petal growth was partially restored by the active gibberellin GA3 or the functional analog of jasmonoyl-isoleucine, coronatine. CYP715 appears to function as a key regulator of flower maturation, synchronizing petal expansion and volatile emission. It is thus expected to be an important determinant of flower-insect interaction

Credits:

Liu Z1, Boachon B1, Lugan R1, Tavares R2, Erhardt M1, Mutterer J1, Demais V3, Pateyron S4, Brunaud V5, Ohnishi T6, Pencik A7, Achard P1, Gong F8, Hedden P8, Werck-Reichhart D9, Renault H10.

  • 1. Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique (CNRS), University of Strasbourg, 67084 Strasbourg, France.
  • 2. Laboratoire de Biométrie et Biologie Évolutive, Université Lyon 1, CNRS, 69622 Villeurbanne, France.
  • 3. Plateforme d'Imagerie In Vitro, IFR 37 de Neurosciences, 67084 Strasbourg, France.
  • 4. Transcriptomic Platform, Unité de Recherche en Génomique Végétale (URGV), INRA, Université d'Evry Val d'Essonne, CNRS, 91057 Evry, France.
  • 5. Bioinformatics for Predictive Genomics, URGV, INRA, Université d'Evry Val d'Essonne, CNRS, 91057 Evry, France.
  • 6. Graduate School of Agriculture, Shizuoka University, Shizuoka, 422-8529 Japan.
  • 7. Laboratory of Growth Regulators & Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University & Institute of Experimental Botany AS CR, 771 47 Olomouc, Czech Republic.
  • 8. Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
  • 9. Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique (CNRS), University of Strasbourg, 67084 Strasbourg, France; University of Strasbourg Institute for Advanced Study (USIAS), 67084 Strasbourg, France; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 79104 Freiburg, Germany. Electronic address: daniele.werck@ibmp-cnrs.unistra.fr.
  • 10. Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique (CNRS), University of Strasbourg, 67084 Strasbourg, France; University of Strasbourg Institute for Advanced Study (USIAS), 67084 Strasbourg, France; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 79104 Freiburg, Germany.

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