InnoScan microarray scanner: new publication

Study Leverages Single-Cell Western Blot to Reveal Drug-Induced Loss of Pancreatic Alpha Cell Identity

Read the new publication using the Innoscan microarray scanner. An inspiring study published in Cell Reports Medicine demonstrates that the antidiabetic drug imeglimin suppresses glucagon secretion and induces transdifferentiation of pancreatic alpha cells into beta-like cells. A key to this discovery was the use of cutting-edge single-cell Western blotting (scWest) technology. The scWest system was crucial for directly detecting and quantifying key proteins like insulin and glucagon simultaneously in hundreds of individual pancreatic cells at single cell level.

Following the scWest processing, the InnoScan 710 microarray scanner provided the essential whole slide high-resolution fluorescence imaging. It precisely measured the fluorescence signal intensity from the antibody-bound protein bands on the specialized chip, enabling the researchers to accurately identify and confirm a population of cells co-expressing the two hormones. The powerful combination of scWest’s single-cell protein resolution and the InnoScan’s sensitive quantification was instrumental in validating this novel mechanism of action, showcasing the critical role of advanced protein analysis tools in driving modern drug discovery.

Keywords:

1. Imeglimin
2. Single-Cell Western Blot (scWestern)
3. Alpha Cell Transdifferentiation
4. Innoscan microarray scanner Imeglimin
5. Glucagon Suppression

Original publication: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells: Cell Metabolism

Learn more about the InnoScan series: InnoScan Microarray Scanners – Innopsys