InnoScan 710-IR - 2-Color near InfraRed microarray scanner
Increased sensitivity
NIR excitation lasers to avoid support background
Simultaneous confocal PMT detection
All nitrocellulose slide formats
Real-time autofocus
Optimized for nitrocellulose or PVDF substrates
Perfect homogeneity across the entire reading surface
Automated image acquisition with MAPIX software
Adjustable reading speed
From 10 to 35 lines per second
Test results optained very quickly 3.6 minutes to read and entire slide with 10μm/pixel
Applications
Technologie
Confocal detection
- Optimize fluorescence detection
- Assure good focalization all along the scan
- Less background
- SNR is increased by 2.5 times
Real-time autofocus
- Detection idone through a spatial phinhole
- Follows the slide movement and deformation in real time during the scan
- Avoid all out-of-focus signal
Automatic barcode reader
- Reliable and traceable results
All standard microscope slides format
- Glass
- Nitrocelluose coated glass
- Plastic
- Silicon wafer
- Gold coated glass
Dynamic range
- From > 104 to >106
- Detect the lowest signals avoiding saturation.
Related documents
Application notes
Publications
Wnt Signaling in Metastatic Cancer
NIR RPPA detection together with dynamic range extension (XDR) function in the InnoScan 710-IR scanner makes RPPA detection cheaper by 90%
InnoScan 710-IR Scanner excels at cytokine detection in conjunction with pGOLD chips
InnoScan 710-IR Infrared detection for optimal scan of nitrocellulose-based protein arrays
Using the infrared increases SNR 3-5 fold of nitrocellulose colorimetric assay-labeled protein microarrays
To amplify or not to amplify: A comparison between fluorescent detection methods for protein microarray applications
Infrared detection increases nitrocellulose autofluorescence and improves RPPA assays signal-to-noise ratio over visible wavelength detection
Reverse Phase Protein Arrays for Cell Signaling: Using the InnoScan 710-IR scanner Improves Sensitivity and Dynamic Range
Technical specifications
InnoScan 710-IR
| PIXEL SIZE | 3 - 40μm | |
| LASER EXCITATION WAVELENGTH | 670nm | 785nm |
| COMPATIBLE FLUOROPHORES* | Alexa Fluor 700, Alexa Fluor 680 DyLight 680, IRDye 680 | Alexa Fluor 790, DyLight 800, IRDye 800 |
| LASER POWER | Adjustable (2 laser powers) | |
| LOADER CAPACITY | 1 slide | |
| DETECTION TYPE | Real-time confocal with 2 photomultipliers (PMT) | |
| PMT GAIN | Adjustable from 0 to 100% (Linear from 1 to 100%) |
|
| OPTICAL FILTER | 1 standard fluorescent filter by detection channel | |
| FOCUS | Real-time autofocus Manual focus: offset adjustment 300 μm range, 1 μm increment |
|
| SLIDE SIZE | Compatible with all standard microscope slides: 25-26 x 75-76 mm2 / 1” x 3” ; thickness: 0.9 - 1.2 mm | |
| SCANNING AREA | Adjustable up to 22 x 74 mm2 | |
| SCANNING SPEED | From 10 to 35 lines/second (l/s) 3.6 minutes per slide at a resolution of 10 μm/pixel for a simultaneous two-color acquisition |
|
| DYNAMIC RANGE | > 104 in normal mode or > 106 in dynamic extension mode | |
| UNIFORMITY | > 95% | |
| BARCODE READER | Automatic barcode reading | |
| INTERFACE | Ethernet interface | |
| IMAGE FORMAT | TIFF (16-bit and 20-bit in dynamic extension mode) | |
| POWER SUPPLY | ~ 100-240 VAC, 1.2 A, 50-60 Hz | |
| ACQUISITION SOFTWARE | MAPIX (image acquisition and spot quantification software) | |
| DIMENSIONS (LXDXH) | 278 x 457 x 369 mm3 10.8” x 18.0” x 14.5” | |
| WEIGHT | 15.5 kg (34.2 lbs) | |
InnoScan 710-IR AL
| LOADER CAPACITY | 24 slides |
| DIMENSIONS (LXDXH) | 322 x 656 x 439 mm3 - 12.7” x 25.8” x 17.3” |
| WEIGHT | 31 kg (62 lbs) |
* Non exhaustive list, please contact us for more information about fluorophore compatibility
Please contact us for any additional information.
Class I laser product for research use only
Specifications subject to change without notice
Contact us for the most recent specifications