InnoScan 710-IR

Technology

Confocal detection

Optimize fluorescence detection
Assure good focalization all along the scan
Less background
SNR is increased by 2.5 times

Real-time autofocus

Detection idone through a spatial phinhole
Follows the slide movement and deformation in real time during the scan
Avoid all out-of-focus signal

Automatic barcode reader

Reliable and traceable results

All standard microscope slides format

Glass
Nitrocelluose coated glass
Plastic
Silicon wafer
Gold coated glass

Dynamic range

From > 10⁴ to >10⁶
Detect the lowest signals avoiding saturation.

Technical specifications

InnoScan 710 IR

PIXEL SIZE	3 - 40μm
LASER EXCITATION WAVELENGTH	670nm	785nm
COMPATIBLE FLUOROPHORES*	Alexa Fluor 700, Alexa Fluor 680 DyLight 680, IRDye 680	Alexa Fluor 790, DyLight 800, IRDye 800
LASER POWER	Adjustable (2 laser powers)
LOADER CAPACITY	1 slide
DETECTION TYPE	Real-time confocal with 2 photomultipliers (PMT)
PMT GAIN	Adjustable from 0 to 100% (Linear from 1 to 100%)
OPTICAL FILTER	1 standard fluorescent filter by detection channel
FOCUS	Real-time autofocus Manual focus: offset adjustment 300 μm range, 1 μm increment
SLIDE SIZE	Compatible with all standard microscope slides: 25-26 x 75-76 mm² / 1” x 3” ; thickness: 0.9 - 1.2 mm
SCANNING AREA	Adjustable up to 22 x 74 mm²
SCANNING SPEED	From 10 to 35 lines/second (l/s) 3.6 minutes per slide at a resolution of 10 μm/pixel for a simultaneous two-color acquisition
DYNAMIC RANGE	> 10⁴ in normal mode or > 10⁶ in dynamic extension mode
UNIFORMITY	> 95%
BARCODE READER	Automatic barcode reading
INTERFACE	Ethernet interface
IMAGE FORMAT	TIFF (16-bit and 20-bit in dynamic extension mode)
POWER SUPPLY	~ 100-240 VAC, 1.2 A, 50-60 Hz
ACQUISITION SOFTWARE	MAPIX (image acquisition and spot quantification software)
DIMENSIONS (LXDXH)	278 x 457 x 369 mm³ 10.8” x 18.0” x 14.5”
WEIGHT	15.5 kg (34.2 lbs)

InnoScan 710 IR AL

LOADER CAPACITY	24 slides
DIMENSIONS (LXDXH)	322 x 656 x 439 mm³ - 12.7” x 25.8” x 17.3”
WEIGHT	31 kg (62 lbs)

* Non exhaustive list, please contact us for more information about fluorophore compatibility
Please contact us for any additional information.

Class I laser product for research use only
Specifications subject to change without notice
Contact us for the most recent specifications

Applications Notes

Wnt Signaling in Metastatic Cancer

NIR RPPA detection together with dynamic range extension (XDR) function in the InnoScan 710-IR scanner makes RPPA detection cheaper by 90%

InnoScan 710-IR Scanner excels at cytokine detection in conjunction with pGOLD chips

InnoScan 710-IR Infrared detection for optimal scan of nitrocellulose-based protein arrays

Using the infrared increases SNR 3-5 fold of nitrocellulose colorimetric assay-labeled protein microarrays

To amplify or not to amplify: A comparison between fluorescent detection methods for protein microarray applications

Publications

Applications

Infectiology

Protein detection by fluorescence-labelled antibodies on tissue sections is a widely used technique for the biomarker screening in complex diseases such as cancer to define biomarker expression on tumor cells and its distribution on a defined tissue.

Reverse Phase Protein Arrays

Reverse Phase Protein Arrays (RPPA), also named cell lysate arrays, have been shown to be a useful tool for biomarker evaluation in large patient cohorts.